Paper Highlights

A nucleobase analogue that pairs strongly with adenine

M. Minuth, C. Richert

Angew. Chem. Int. Ed. 2013, DOI:/10.1002/anie.201305555

Adenin war bisher schwer zu binden. Ein 6-Ethinyl-2-pyridon-C-Nucleosid paart mit hoher Affinität und Selektivität mit A in DNA- und RNA-Zielsträngen.

 

Specific DNA duplex formation at an artificial lipid bilayer: Towards a new DNA biosensor technology

Emma Werz, Sergei Korneev, Malayko Montilla-Martinez, Richard Wagner, Roland Hemmler, Claudius Walter, Jörg Eisfeld, Karsten Gall, Helmut Rosemeyer

Chem. Biodiversity 2012, DOI:10.1002/cbdv.201100298

First experiments towards a novel DNA chip technology are presented which renounces any chemistry on solid supports by using instead the self-organization and duplex formation of lipid-oligonucleotide conjugates at a lipid bilayer – water interface.

 

An RNA–deaminase conjugate selectively repairs point mutations

T. Stafforst, M. F. Schneider

Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201206489

Checking for mistakes: By conjugating a catalytic domain with a guide RNA, deamination activity can be harnessed to repair a specific codon on mRNA. This method can be used for the highly selective repair of point mutations in mRNA by site-selective editing.

 

Organic chemistry of DNA functionalization; chromophores as DNA base substitutes versus DNA base/2'-modifications

W. Schmucker, H. A. Wagenknecht

Synlett 2012, DOI:10.1055/s-0032-1317158

Chemical synthesis allows to use nucleic acids as scaffolds for the precise arrangement of different kinds of artificial functionalities inside or along the double helix.

 

Template-directed synthesis in 3′- and 5′-direction with reversible termination

A. Kaiser, S. Spies, T. Lommel, C. Richert

Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201203859

Ein Verfahren für die templatgesteuerte Festphasensynthese von DNA mit Phosphorsäureamid-Rückgrat wird beschrieben.
Kettenwachstum kann am 3'- und am 5'-Terminus induziert werden.

 

In-stem-labeled molecular beacons for distinct fluorescent color readout

C. Holzhauser, H.-A. Wagenknecht

Angew. Chem. Int. Ed. 2011, DOI:10.1002/anie.201101968

DNA traffic lights for hybridization: An efficient energy transfer between thiazole orange and thiazole red in the DNA stem architecture yields a red fluorescence that changes to green emission upon binding to the target oligonucleotide sequence.