M. Minuth, C. Richert
Angew. Chem. Int. Ed. 2013, DOI:/10.1002/anie.201305555
Adenin war bisher schwer zu binden. Ein 6-Ethinyl-2-pyridon-C-Nucleosid paart mit hoher Affinität und Selektivität mit A in DNA- und RNA-Zielsträngen.
Emma Werz, Sergei Korneev, Malayko Montilla-Martinez, Richard Wagner, Roland Hemmler, Claudius Walter, Jörg Eisfeld, Karsten Gall, Helmut Rosemeyer
Chem. Biodiversity 2012, DOI:10.1002/cbdv.201100298
First experiments towards a novel DNA chip technology are presented which renounces any chemistry on solid supports by using instead the self-organization and duplex formation of lipid-oligonucleotide conjugates at a lipid bilayer – water interface.
T. Stafforst, M. F. Schneider
Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201206489
Checking for mistakes: By conjugating a catalytic domain with a guide RNA, deamination activity can be harnessed to repair a specific codon on mRNA. This method can be used for the highly selective repair of point mutations in mRNA by site-selective editing.
W. Schmucker, H. A. Wagenknecht
Synlett 2012, DOI:10.1055/s-0032-1317158
Chemical synthesis allows to use nucleic acids as scaffolds for the precise arrangement of different kinds of artificial functionalities inside or along the double helix.
A. Kaiser, S. Spies, T. Lommel, C. Richert
Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201203859
Ein Verfahren für die templatgesteuerte Festphasensynthese von DNA mit Phosphorsäureamid-Rückgrat wird beschrieben.
Kettenwachstum kann am 3'- und am 5'-Terminus induziert werden.
C. Holzhauser, H.-A. Wagenknecht
Angew. Chem. Int. Ed. 2011, DOI:10.1002/anie.201101968
DNA traffic lights for hybridization: An efficient energy transfer between thiazole orange and thiazole red in the DNA stem architecture yields a red fluorescence that changes to green emission upon binding to the target oligonucleotide sequence.
