Christoph Kröner, Martin Thunemann, Sven Vollmer, Manuela Kinzer, Robert Feil, Clemens Richert
Angew. Chem. Int. Ed. 2014, DOI:10.1002/anie.201403579
A de novo designed RNA motif binds cGMP with low micromolar affinity and, when inducibly expressed in cells, acts as sink for this second messenger.
H. S. Steinert, F. Schäfer, H. R. A. Jonker, A. Heckel, H. Schwalbe
Angew. Chem. Int. Ed. 2014, DOI:10.1002/anie.201307852
Photolabile protecting groups are a versatile tool to trigger reactions by light irradiation. In this study, we have investigated the influence of the absolute configuration of the 1-(2-nitrophenyl)ethyl (NPE) cage group on a 15-base-pair duplex DNA. Using UV melting, we determined the global stability of the unmodified and the selectively (S)- and (R)-NPE-modified DNA sequences, respectively. We observe a differently destabilizing effect for the two NPE stereoisomers on the global stability. Analysis of the temperature dependence of imino proton exchange rates measured by NMR spectroscopy reveals that this effect can be attributed to decreased base pair stabilities of the caged and the 3′-neighbouring base pair, respectively. Furthermore, our NMR based structural models of the modified duplexes provide a structural basis for the distinct effect of the (S)- and the (R)-NPE group.
F. Rohrbach, F. Schäfer, M. A. H. Fichte, F. Pfeiffer, J. Müller, B. Pötzsch, A. Heckel, G. Mayer
Angew. Chem. Int. Ed. 2013, DOI:10.1002/anie.201306686
Photolabile protecting groups are a versatile tool to trigger reactions by light irradiation. In this study, we have investigated the influence of the absolute configuration of the 1-(2-nitrophenyl)ethyl (NPE) cage group on a 15-base-pair duplex DNA. Using UV melting, we determined the global stability of the unmodified and the selectively (S)- and (R)-NPE-modified DNA sequences, respectively. We observe a differently destabilizing effect for the two NPE stereoisomers on the global stability. Analysis of the temperature dependence of imino proton exchange rates measured by NMR spectroscopy reveals that this effect can be attributed to decreased base pair stabilities of the caged and the 3′-neighbouring base pair, respectively. Furthermore, our NMR based structural models of the modified duplexes provide a structural basis for the distinct effect of the (S)- and the (R)-NPE group.
M. Minuth, C. Richert
Angew. Chem. Int. Ed. 2013, DOI:/10.1002/anie.201305555
Adenin war bisher schwer zu binden. Ein 6-Ethinyl-2-pyridon-C-Nucleosid paart mit hoher Affinität und Selektivität mit A in DNA- und RNA-Zielsträngen.
Emma Werz, Sergei Korneev, Malayko Montilla-Martinez, Richard Wagner, Roland Hemmler, Claudius Walter, Jörg Eisfeld, Karsten Gall, Helmut Rosemeyer
Chem. Biodiversity 2012, DOI:10.1002/cbdv.201100298
First experiments towards a novel DNA chip technology are presented which renounces any chemistry on solid supports by using instead the self-organization and duplex formation of lipid-oligonucleotide conjugates at a lipid bilayer – water interface.
T. Stafforst, M. F. Schneider
Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201206489
Checking for mistakes: By conjugating a catalytic domain with a guide RNA, deamination activity can be harnessed to repair a specific codon on mRNA. This method can be used for the highly selective repair of point mutations in mRNA by site-selective editing.
