Paper Highlights

Aptamer-guided caging for selective masking of protein domains

F. Rohrbach, F. Schäfer, M. A. H. Fichte, F. Pfeiffer, J. Müller, B. Pötzsch, A. Heckel, G. Mayer

Angew. Chem. Int. Ed. 2013, DOI:10.1002/anie.201306686

Photolabile protecting groups are a versatile tool to trigger reactions by light irradiation. In this study, we have investigated the influence of the absolute configuration of the 1-(2-nitrophenyl)ethyl (NPE) cage group on a 15-base-pair duplex DNA. Using UV melting, we determined the global stability of the unmodified and the selectively (S)- and (R)-NPE-modified DNA sequences, respectively. We observe a differently destabilizing effect for the two NPE stereoisomers on the global stability. Analysis of the temperature dependence of imino proton exchange rates measured by NMR spectroscopy reveals that this effect can be attributed to decreased base pair stabilities of the caged and the 3′-neighbouring base pair, respectively. Furthermore, our NMR based structural models of the modified duplexes provide a structural basis for the distinct effect of the (S)- and the (R)-NPE group.

 

A nucleobase analogue that pairs strongly with adenine

M. Minuth, C. Richert

Angew. Chem. Int. Ed. 2013, DOI:/10.1002/anie.201305555

Adenin war bisher schwer zu binden. Ein 6-Ethinyl-2-pyridon-C-Nucleosid paart mit hoher Affinität und Selektivität mit A in DNA- und RNA-Zielsträngen.

 

Specific DNA duplex formation at an artificial lipid bilayer: Towards a new DNA biosensor technology

Emma Werz, Sergei Korneev, Malayko Montilla-Martinez, Richard Wagner, Roland Hemmler, Claudius Walter, Jörg Eisfeld, Karsten Gall, Helmut Rosemeyer

Chem. Biodiversity 2012, DOI:10.1002/cbdv.201100298

First experiments towards a novel DNA chip technology are presented which renounces any chemistry on solid supports by using instead the self-organization and duplex formation of lipid-oligonucleotide conjugates at a lipid bilayer – water interface.

 

An RNA–deaminase conjugate selectively repairs point mutations

T. Stafforst, M. F. Schneider

Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201206489

Checking for mistakes: By conjugating a catalytic domain with a guide RNA, deamination activity can be harnessed to repair a specific codon on mRNA. This method can be used for the highly selective repair of point mutations in mRNA by site-selective editing.

 

Organic chemistry of DNA functionalization; chromophores as DNA base substitutes versus DNA base/2'-modifications

W. Schmucker, H. A. Wagenknecht

Synlett 2012, DOI:10.1055/s-0032-1317158

Chemical synthesis allows to use nucleic acids as scaffolds for the precise arrangement of different kinds of artificial functionalities inside or along the double helix.

 

Template-directed synthesis in 3′- and 5′-direction with reversible termination

A. Kaiser, S. Spies, T. Lommel, C. Richert

Angew. Chem. Int. Ed. 2012, DOI:10.1002/anie.201203859

Ein Verfahren für die templatgesteuerte Festphasensynthese von DNA mit Phosphorsäureamid-Rückgrat wird beschrieben.
Kettenwachstum kann am 3'- und am 5'-Terminus induziert werden.